Fields and Living Matter Neoplastic CelIuIar Culture
Fusion (Grade 1)
figures 1 through 5 the effects on neoplastic HeLa cells in contrast phase
can be seen through the microscope. The culture was exposed to
electromagnetic waves with a frequency in the megahertz range and a power of
0.25 watts for a period of about three hours. The electromagnetic energy
modulated in this way brings about cytoplasmic celi fuion,
which produces up to a maximum of five cells, after which cell necrosis
In these figures the approach of the two cell structures, located in
the center of the picture, can be seen until their fusion occurs. In figure
3 the membranes come into contact at which point the potential cell alteration can be noted (Grade 1). The
above phenomenon was first noticed
in 1970 and has been repeated a number of times; it was also reported at the
Balcanico International Congress of 1979.
Fusion and Necrosis (Grade 2)
6 through 9 indicate the progressive fusion and necrosis in vitro of cancer
cells of the CC-178 line.
These observations were conducted by the
Department of Hematology and Oncology at the University of Hanover by sub
jecting the cells to electromagnetic waves with frequencies in the
megahertz range at a power of 0.25 watts for a period of about two hours.
of Electromagnetic Fields on Cell Functions
preliminary observations conducted in vitro show an alteration of cell morphology, a halt to proliferation, fusion, and necrosis in
lymphoblastoid cell lines and in some neoplastic lines, after treatment with specificaliy modulated electromagnetic fieids (HeLa, mammary carcinoma,
CCL-178, colon adenocarcinoma, H 23, H 32, h 12.1, 1411 H, testicle carcinoma,
M 5, MSi, stomach carcinoma, MCF-7 human Caucasian breast adenocarcinoma
ECACC 86012803, normal cell line, and MDBK bovine kidney cells) (16).
is known that cells communicate with each other by means of direct
metabolic exchanges or through the transfer of ions or molecules that act as
messengers. Multicell signals which originate in the interaction of ligands
with membrane receptors can activate a closely connected series of biochemical
reactions. The biological membranes represent multimolecular
operative structures, and even a slight alteration in the composition of
the membrane can Iead to significant changes in its functions.
Electromagnetic fields can infiuence this communication between cells and
within the cells themselves due to their ability to activate or change the
motion of the electricai charges.
In fact, an increasing amount of
literature iilustrates the possibility of inducing biological effects in
cells when appropriate electrical and magnetic fields are applied to have
a direct effect on the membranes
(94, 95, 96, 97).
the various effects obtained are those on Na+ and K+ dynamics and their
role in ATPasi, as well as the effects on the intermembrane
exchanges of the Ca++ ion, which, because of its presence in most
biomolecular processes, has earned the name of second messenger (94).
Moreover, exposure conditions that have led to effects on the membrane permeabiiity
of the Ca++ ion have shown a negative influence on the mitotic fuso, and
this influence is selectively tied to the characteristics of the
magnetic field used. Up to now, the results obtained imply that the
membrane receptors (e.g., the glucoprotein complexes), are able to decipher
electrical signals at a well defined frequency and amplitude by reacting
in a specific way. The energy transformed from the electrical field is
absorbed and directly coupled to guide biochenucal reactions.
results have served as the bases for some applications in the therapeutic
field, particularly in the reproduction of bone tissue. (98) This is due
to the fact that the activation of some cell functions is bound to
eiectrical potentials of the on/off type, that is, not with linear but with
Cell Fusion and Necrosis (Grade 2)
possibility that weak electric or magnetic fields can send signals past
the strong potential barrier of the cytoplasmic membrane (100 KV/cm) can
be explained by the hypothesis of the phenomena of resonance on certain
kinds of ions (101), the cooperative gapjunction type phenomena (102,
103), and the amplification effects explained by the set up of a field
gradient between the inside and outside of a spherical shell made up of
three layers of dielectric properties (95). The treated cells were examined
with an electron microscope that showed ultrastructural alterations in the
Cytoskeleton fiber—at the structure alteration Ievel with an
inaease in fibers compared to the control and with a more irregular disposition and orientation
Mitochondrion—a different orientation of the mitochondrion crests
and an alteration of the mitochondrion matrix which appears dishomogeneous and pycnotic compared to the control
Autophages— intra-cytoplasmic bodies in many cells
the following can be noted:
Thickening of the chromatin at the nuclear membrane level
These types of alterations,
especially at the nuclear level, suggest the hypothesis that an apoptotic
type of phenomenon was induced by the treatment.
The characteristics of the
equipment for these studies was as follows: 10w power (0.25 watts) electromagnetic
waves with frequencies in the kilohertz range and magnetic fields and
electrostatic fields specifically modulated according to the Gorgun method
(GEMM: Modulated electromagnetic generator).
Pag. 8 -